【摘 要】
：
During intracellular membrane trafficking, N-ethylmaleimide-sensitive factor (NSF) and alpha-soluble NSF attachment protein (a-SNAP) disassemble the soluble NSF attachment protein receptor (SNARE) com
【机 构】
：
Physics, KAIST, Daejeon, South Korea Chemistry an
【出 处】
：
The 9th Asian Biophysics Association Symposium (ABA2015)(第九届
论文部分内容阅读
During intracellular membrane trafficking, N-ethylmaleimide-sensitive factor (NSF) and alpha-soluble NSF attachment protein (a-SNAP) disassemble the soluble NSF attachment protein receptor (SNARE) complex for recycling of the SNARE proteins.The molecular mechanism by which NSF disassembles the SNARE complex is largely unknown.Using single-molecule fluorescence spectroscopy and magnetic tweezers, we found that NSF disassembled a single SNARE complex in only one round of adenosine triphosphate (ATP) turnover.Upon ATP cleavage, the NSF hexamer developed internal tension with dissociation of phosphate ions.After latent time measuring tens of seconds, NSF released the built-up tension in a burst within 20 milliseconds, resulting in disassembly followed by immediate release of the SNARE proteins.Thus, AAA+ adenosine triphosphatases (ATPases) appear to use a "spring-loaded" mechanism to couple ATP hydrolysis and unfolding of substrate proteins.
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