【摘 要】
：
Monoclonal antibodies (MAbs) against pyrethroid insecticide fenvalerate were achieved, identified, and applied in environmental water.Mice were immunized with a novel synthesized hapten conjugated wit
【机 构】
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Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan,China;Quality Inspection
【出 处】
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中国作物学会油料作物专业委员会第七次会员代表大会暨学术年会
论文部分内容阅读
Monoclonal antibodies (MAbs) against pyrethroid insecticide fenvalerate were achieved, identified, and applied in environmental water.Mice were immunized with a novel synthesized hapten conjugated with bovine serum albumin (BSA).Three positive clones of MAbs were obtained after cell fusion and hybridoma selection, among them MAb-2 (5B10) showed the highest reactivity toward fenvalerate.The IC50 of MAb-2 was 94.5 ng· mL-1; moreover, it showed lower cross-reactivity with other pyrethroids such as bifenthrin, tetramethrin, deltamethrin and beta-cypermethrin.Optimization of enzyme-linked immunosorbent assay (ELISA) was studied.The limit of detection (LOD) of the assay was 8.8ng · mL-1 and the detection range was 0.017-27.33μg ·mL-1.For preliminary application, addition recovery experiments in water samples were performed.The mean recoveries of three kinds of samples varied from 90.6% to 108.7% and the coefficients of variation ranged from 0.5% to 5.3%.The results showed that MAb-2 could be used for the detection of fenvalerate contamination in environmental water.
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