Determination of both Jasmonic Acid and Methyl Jasmonate in Plant Samples by Liquid Chromatography T

来源 :第八届海峡两岸三地植物分子生物学与生物技术学术研讨会 | 被引量 : 0次 | 上传用户:tomyang
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  An efficient method for the extraction and quantification of both jasmonic acid (JA) and methyl jasmonate (MeJA) from a single plant sample was developed.Plant tissues were firstly extracted using 100% cold methanol, then the Sep-pak C18 solid-phase extraction (SPE) cartridges were used for the purification of sample extract, and finally JA and MeJA were determined by the liquid chromatography tandem mass spectrometry(LC-MS-MS) system.
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