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To determine the Effects of xylanase, lactobacillus and xylo-oligosaccharide on intestinal organs and tissue development of broiler chickens from 1-63 days, intestinal morphometry, total bacterial DNA count and Gene expression of TJP were evaluated.The 5120, 1 day old Yellow-feather broiler chicks were randomly allotted to 8 treatments with 5 replications per treatment.The treatments includes: (A) Basal diets as Control;(B) Control + Enzymes 100g/ton;(C) Control + Probiotics 200g/ton;(D) Control + Prebiotics 100g/ton;(E) Control + Enzymes +Probiotics;(F) Control + Enzymes + Prebiotics;(G) Control + Enzymes + Probiotics + Prebiotics;and (H) Control + Probiotics + Prebiotics.