目的 致病菌的即时检测对于感染性疾病的现场快速诊断具有重要意义.基于PCR的分子诊断技术不适于现场即时核酸检测.本文建立了基于均相靶识别诱导原位转录和电化学传感的分子检测新方法,用于致病菌的简单快速鉴定.方法 设计针对沙门氏菌InvA基因的多功能发夹探针,在同一检测体系中的均相环境完成靶基因的特异识别结合,保证了靶识别结合的高亲和力和均一性;在固相表面完成灵敏的电化学检测,保持了固相传感器的空间分
目的 布鲁氏菌病是由布鲁氏菌(Brucella spp,Bru)感染引起的一种严重人兽共患传染病,人在接触患病动物或被污染的动物产品后即可发病,世界上每年布鲁氏菌病发病人数超过50万.在中国,布鲁氏菌病一直是危害人民健康的公共卫生问题,自1999年以来,中国布鲁氏菌病发病率呈现不断增加的趋势和特定地域发病的特点,发病的地域与聚集牛羊等牲畜的草原有密切的空间相关性.新疆地区紧邻中亚,因畜牧业发达、饮
Conventional chemotherapeutics and targeted antineoplastic agents have been developed based on the simplistic notion that cancer constitutes a cell-autonomous genetic or epigenetic disease.Nonetheless
Toll-like receptors(TLRs) play key roles in the innate immune respons to defense invading microbial pathogens.The identification of the negative regulators and details of the mechanisms by which TLR s
Tuberculosis (TB) remains one of the leading infectious diseases worldwide.L-forms (also called cell wall deficient forms) Mycobacterium tuberculosis (Mtb), which are responsible for persistent infect
Macrophage play critical roles in renal fibrosis, but the mechanisms of macrophages activation and function in renal fibrosis has not been fully understood.Literatures and our previous studies have sh
Immunosuppression is the main source of ineffective treatment on tumor, and the study aimed to investigate the effect of artesunate on tumor immunosuppression.Supematants of re-cultivated Colon26 (mur
Objective: Recently, apoptosis clearance has been proposed to involve in the pathophysiology of chronic obstructive pulmonary disease (COPD).It has been suggested that Milk fat globule-EGF factor 8 (M
The NLRP3 inflammasome is a cytosolic protein complex composed of NLRP3, ASC and caspase-1,and assembled in response to both microbial infection and endogenous "danger signal".The activation of NLRP3
Objective: Cultured of primary vascular endothelial cells from human umbilical veins endothelial cells (HUVECs) and simulation of substrate stiffness about HUVECs.To observe inoculated of primary HUVE