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为了研究“髓复康”在体外培养中是否具有一定的神经元保护作用 ,本研究采用原代培养胎鼠脊髓神经元 ,分别将高浓度的髓复康 ( 10μl/ ml)、低浓度的髓复康 ( 5μl/ ml)与体外培养 5 d的原代脊髓神经元共同孵育 2 4h,空白对照组不加中药只加入等量的培养液。选用 10 0 μmol/ L的谷氨酸作用于细胞 1h,建立谷氨酸诱导原代脊髓神经元凋亡的细胞培养模型 ,采用酶学方法( MTT法 )评价神经元损伤程度。结果表明 ,髓复康组神经元线粒体内的琥珀酸脱氢酶的活性明显地高于空白对照组 ,组间有极显著的差异 ( P<0 .0 0 1)。提示 ,“髓复康”可以部分地抑制谷氨酸的兴奋性神经毒性作用
In order to study whether “Pulp” has a certain neuroprotective effect in vitro culture, primary cultured rat fetal spinal cord neurons were used in this study. High concentrations of pilospirillin (10 μl/ml) and low concentrations of pith were used. Recombinant (5μl/ml) was incubated with primary spinal cord neurons cultured in vitro for 24 hours. The blank control group received only the same amount of culture medium without Chinese medicine. 100 μmol / L glutamate was applied to the cells for 1 h to establish a cell culture model for glutamate-induced apoptosis of primary spinal neurons. The extent of neuronal damage was assessed by enzymatic method (MTT). The results showed that the activity of succinate dehydrogenase in the mitochondria of the spinal cord in the Pycnogenol group was significantly higher than that in the blank control group, and there was a significant difference between the groups (P<0.01). Prompt, “Puduukang” can partially inhibit glutamate excitatory neurotoxicity