昆虫的几丁质酶家族group I主要负责蜕皮过程中表皮几丁质降解,被认为是农药设计的潜在靶标.通过分析亚洲玉米螟几丁质酶OfChtI催化域的晶体结构,发现其活性口袋底物结合裂缝处存在一系列芳香族氨基酸,提供其与底物结合的关键疏水作用,同时也是基于结构进行选择性抑制剂筛选的重要靶点.基于以上发现,通过对比底物几丁三糖化合物结构、电荷性质对商品化合物数据库进行虚拟筛选,获得了其抑制剂TP1,TP2.
目前对生物大分子的定量研究主要采用“绝对定量”和“相对定量”的方式。但是，由于研究对象—生物体的多样性、动态性和复杂性，导致了以往研究所采用的定量体系已经不能满足于大数据时代分子生物学研究的需要。对于原始数据的产出，分子生物学研究亟需在方法学上建立新的理论体系，新的生物大分子定量规范和新的生物样本定量规范需要重新建立。在新的生物定量模式下开展定量分子生物学研究将是分子生物学的发展趋势。
Bacteria have been known to be able to enter a non-growing state when encountering unfit conditions,termed persister.They take a variable lag time and exhibit multidrug tolerance before resuming growt
Metabolic reprogramming greatly contributes to the regulation of macrophage activation.However,the mechanism of lipid accumulation and the corresponding function in tumor-associated macrophages(TAMs)r
The Eph receptor tyrosine kinase(RTK)family is the largest subfamily of RTKs playing critical roles in many developmental processes such as tissue patterning,neurogenesis and neuronal circuit formatio
Pericardial adipose tissue,which comprises both epicardial adipose tissue(EAT)and paracardial adipose tissue(PAT),has recently been recognized as a novel factor in the pathophysiology of cardiovascula
Analysis of blood-based glycans represents a new strategy for cancer diagnosis.Immunoglobin G(IgG),a highly abundant glycoprotein in serum,is known to mediate a variety of blood immune responses.There
Single-molecule techniques have become an indispensable tool for studying the dynamics of bio-molecules and biomembranes.Here,we survey important recent development in this field of research and provi
TIFA(TRAF-interacting protein with forkhead-associated domain)is a small FHA-containing human protein.It was first identified as a TRAF2 binding protein and later reported to also bind to TRAF6 and pl
毛发缺失给患者的心理造成了极大的困扰。对于增强毛发再生能力的研究有助于毛发缺失的治疗。血管内皮生长因子(VEGF)，又称血管通透因子，是一种高度特异性的促血管内皮细胞生长因子，具有促进血管通透性增加、细胞外基质变性、血管内皮细胞迁移、增殖和血管形成等作用，可促进靶组织的血管生成，促进局部组织修复。干细胞因子(SCF)，又称肥大细胞生长因子，是由骨髓微环境中的基质细胞产生的一种酸性糖蛋白，和其他细胞