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目的探讨帕金森病(PD)猴模型丘脑底核(STN)神经元的电生理特性,为研究帕金森病的病理生理过程提供动物实验依据。方法应用颈内动脉注射MPTP建立PD猴模型。在立体定向仪引导下应用细胞外记录的方法记录“造模”前后猴病理及正常生理状态下STN神经元的放电活动,并对其放电模式进行分析。结果电生理记录显示STN神经元放电频率在生理状态下为2.03±1.12Hz;在病理状态下为9.58±0.85 Hz(P<0.01)。在生理状态下有20个(20/35,57.14%)神经元呈现簇发放电,有15个(15/35,42.86%)神经元呈现连续放电;在PD病理状态下有10个(10/12,85.71%)神经元呈现簇发放电,有2个(2/12,14.29%)神经元呈现连续放电(P<0.05)。生理状态下STN神经元的ISI序列散在分布于30～980ms之间;在PD病理状态下当STN神经元呈连续放电时,ISI分布于50～360ms之间,在150ms以下有一个分布密集条带,当STN神经元呈簇发放电时,ISI分布于30～470ms之间,在40ms以下有一个分布密集条带。结论PD猴模型STN神经元较生理状态下放电频率明显增加,其放电模式也有明显变化,簇状放电模式比例增大,ISI序列发生明显变化。
Objective To investigate the electrophysiological characteristics of subthalamic nucleus (STN) neurons in Parkinson’s disease (PD) monkeys and provide animal experimental evidences for studying the pathophysiological process of Parkinson’s disease. Methods The MPTP model was established by injecting MPTP into the internal carotid artery. Under the guidance of stereotactic apparatus, extracellular recording method was used to record the discharge activity of STN neurons in both pathological and normal physiological states before and after the “modeling” and the discharge patterns were analyzed. Results Electrophysiological records showed that the discharge frequency of STN neurons was 2.03 ± 1.12 Hz in physiological state and 9.58 ± 0.85 Hz in pathological state (P <0.01). In the physiological state, 20 (20/35, 57.14%) neurons showed cluster-like discharge and 15 (15/35, 42.86%) neurons showed continuous discharge; in the PD pathological state, 10 12,85.71%) neurons showed cluster-like discharge, and 2 (2 / 12,14.29%) neurons showed continuous discharge (P <0.05). ISI sequences of STN neurons were distributed randomly between 30 and 980 ms under physiological conditions. When STN neurons were continuously discharged under pathological conditions of PD, ISI was distributed between 50 and 360 ms, with a dense distribution below 150 ms When the STN neurons were clustered and discharged, the ISI was distributed between 30 and 470ms, and there was a densely distributed band below 40ms. Conclusions The frequency of discharge in STN neurons of PD monkey model is significantly increased compared with that in physiological state, and the discharge patterns also change obviously. The proportion of cluster discharge patterns increases and the sequence of ISI changes significantly.