论文部分内容阅读
[目的]建立发病机制与人类相似的2型糖尿病并脂肪肝的动物模型。[方法]Wistar大鼠随机分为正常组、链脲佐菌素(STZ)25 mg/kg加高脂饲料组(先注射STZ后喂以高脂饲料)、STZ 12.5 mg/kg加高脂饲料组、高脂饲料加STZ 25 mg/kg组(先喂以高脂饲料后注射STZ)、高脂饲料加STZ 12.5 mg/kg组。10周后,测动物血糖、血脂、肝功能、胰岛素;取肝切片,苏木精-伊红染色、苏丹三醇染色。[结果]STZ 25 mg/kg加高脂饲料组动物血糖、血脂、肝功能、胰岛素抵抗指数有显著的变化,组织病理学也有典型脂肪肝变化。[结论]小剂量STZ注射加高脂饲料喂养可以建立2型糖尿病并脂肪肝动物模型。
[Objective] To establish an animal model of type 2 diabetes mellitus and fatty liver whose pathogenesis is similar to that of human. [Methods] Wistar rats were randomly divided into normal group, STZ 25 mg / kg plus high fat diet group (STZ high fat diet), STZ 12.5 mg / kg plus high fat diet Group, high fat diet plus STZ 25 mg / kg group (fed with high fat diet before STZ injection), high fat diet plus STZ 12.5 mg / kg group. After 10 weeks, the blood glucose, blood lipids, liver function and insulin in animals were measured. The liver sections were taken and stained with hematoxylin and eosin. [Result] The blood glucose, blood lipid, liver function and insulin resistance index of STZ 25 mg / kg plus high-fat diet group had significant changes, and histopathology also had typical fatty liver changes. [Conclusion] Low-dose STZ injection plus high-fat diet can establish type 2 diabetic and fatty liver animal model.