【摘 要】
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OBJECTIVE This study was conducted to establish a high-throughput screening method of cell transformation assay in Bhas 42 cells, aiming to shorten the experimental period and increase the level of ob
【机 构】
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National Center for Safety Evaluation of Drugs, National Institute for Food and Drug Control, Beijin
【出 处】
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2013年(第三届)中国药物毒理学年会暨药物非临床安全性评价研究论坛
论文部分内容阅读
OBJECTIVE This study was conducted to establish a high-throughput screening method of cell transformation assay in Bhas 42 cells, aiming to shorten the experimental period and increase the level of objectivity of in vitro cell transformation assay in the detection of carcinogens and to study the transformation potential of genistein.METHODS (① Assay Establishment: Two cell transformation methods were established in our lab for the Bhas 42 cell transformation assay: traditional foci formation method and hydrogen peroxide treatment method.
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