【摘 要】
：
Polar zooplankton are particularly sensitive to climate change, and have been used as rapid-responders to indicate climate-induced changes in the fragile Antarctic ecosystem.DNA barcoding provides an
【机 构】
：
Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academ
论文部分内容阅读
Polar zooplankton are particularly sensitive to climate change, and have been used as rapid-responders to indicate climate-induced changes in the fragile Antarctic ecosystem.DNA barcoding provides an alternative approach for rapid zooplankton species identification.Ninety-four specimens belonging to 32 Antarctic zooplankton species were barcoded to construct a comprehensive reference library.An 830 to 1 050 base-pair region of the mitochondrial cytochrome c oxidase subunit I (mtCOI) gene was obtained as DNA barcodes.The intraspecific variation of the gene ranged from 0 to 2.6% (p-distance), with an average of 0.67% (SD=0.67%).The distance between species within the same genera ranged from 0.1% (Calanus) to 29.3%, with an average of 15.3% (SD=8.4%).The morphological and genetic similarities between Calanus propinquus and C.simillimus raise new questionsabout the taxonomic status of C.simillimus.With the exception of the two Calanus species, the intraspecific genetic divergence was much smaller than the interspecific divergence among congeneric species, confirming the existence of a barcode gap for Antarctic zooplankton.In addition, species other than Calanus sp.formed a monophyletic group.Therefore, we have confirmed DNA barcoding as an accurate and efficient approach for zooplankton identification in the Antarctic area (except for Hydromedusa, Tunicata, and other gelatinous zooplankton).Indicator vector analysis further confirmed this conclusion.The new primer sets issued here may facilitate the study of Antarctic marine zooplankton species composition by environmental metagenetic analysis.
其他文献
To research the structure radiation stability of simulated Gd2Zr2O7-pyrochlore glass-ceramic wasteforms Nd3+, Ce4+ were selected as the simulated nuclide of An3+ and An4+ radionuclides respectively.A
The main aim of this work is to study the possibility of using different Emirati limestones for the production of quicklime.Representative limestone samples have been collected to represent the Triass
The flake-like basic magnesium carbonate was prepared from the ammonium sulfate leaching liquid of asbestos tailings.The method of X-ray diffraction, Fourier transform infrared spectra, TA-DTG and SEM
The Lop Nur playa is located in the eastern part of the Tarim Basin, Xinjiang.From the late Middle Pleistocene to the Late Pleistocene, the Lop Nur gradually evolved into a salt lake.Huge amounts of g
Tubular halloysite is a hydrated polymorph of kaolinite, commonly considered as its associated mineral.Due to its unique mesoscopic (2-50 nm) tubular structure, it has attracted great interest in the
The experimental prediction of the mechanical properties of minerals remains difficult because of their small particle size (typically ＜ 2 micrometers).This work aims at examining, the effect of an ap
In this paper, a high calcium high sulfate ash as the main material, adding fly ash, lime, cement, gypsum and some modifiers to prepare autoclaved aerated concrete.The products complies with the techn
Talc is a hydroxyled, magnesium-rich, layered silicate that has the ideal chemical formula Mg3Si4O10(Oh)2.Due to its unique properties, such as softness, chemical inermess, high thermal stability, low
Chemical and mineralogical characterizations of high Ti-bearing blast furnace slag in Panzhihua region were investigated and the technical approach "recycling Fe by magnetic separation-extracting Al b
Formation and dissolution of secondary arsenic minerals often play significant roles in controlling arsenic mobility in contaminated environments, especially in sulfide mines.Weathering of the orpimen