【摘 要】
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To study the antiviral activity of natural herbs to porcine reproductive and respiratory syndrome virus (PRRV),anti-viral effect of Indigo Naturalis,Sichuan pepper,euonymus and aloes were analyzed by
【机 构】
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College of Animal Science and Technology, Yunnan Agricultural University,Kunming 650201,China
【出 处】
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2013国际猪繁殖与呼吸综合征学术研讨会
论文部分内容阅读
To study the antiviral activity of natural herbs to porcine reproductive and respiratory syndrome virus (PRRV),anti-viral effect of Indigo Naturalis,Sichuan pepper,euonymus and aloes were analyzed by using in vitro cell culture system model.Based on detection of median toxic concentration (TC50) and maximal nontoxic concentration (TC0) of four herbal extracts by CCK-8 method,the cytopathic effect was observed and virus nucleoprotein gene (ORF7) mRNA expression determined real-time quantitative RT-PCR.Results of cytotoxicity and virus proliferation inhibition analysis shows that antiviral selection index (SI) of Indigo Naturalis,Sichuan pepper,Euonymus and Indigo naturalis were respectively 10.3,5.2,12.7 and 6.Besides,mRNA expression level of viral NP protein gene (ORF7) determined by real-time PCR were significantly inhibited within certain range of concentration.Results of this study confirm that extracts of four herbals have obvious anti-PRRSV effects,the best effect found in extract of Euonymus.
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