【摘 要】
：
MicroRNAs are small RNAs that regulate gene expression at the post-transcriptional level, which always showed abnormally expressed in cancer.We found that miR-128 is abnormally expressed in colon canc
【机 构】
：
Department of physiology,basic medical school,Wuhan university,Wuhan,430071 China
【出 处】
：
International Conference for Physiological Sciences 2012(201
论文部分内容阅读
MicroRNAs are small RNAs that regulate gene expression at the post-transcriptional level, which always showed abnormally expressed in cancer.We found that miR-128 is abnormally expressed in colon cancer, but the role of miR-128 in colon cancer still keeps indistinct.Compared with absent tissue, the miR-128 level decreased in colon cancer clinical samples by qPCR assay.Overexpression of miR-128 could inhibit cell growth and decreased chemosensitivity to methotrexate in HCT116.Flow cytometry assay showed miR-128 induced cell cycle arrest by decreasing S phase.We found that prohibitin (PHB) is one of the targets of miR-128, Western blotting also showed that miR-128 could decrease PHB protein expression level, increase P53 and P21 protein levels.We also found that miR-128 could inhibit migration in colon cancer cell line, the mechanism need more intensively work.Our work showed that miR-128 could induce cell cycle arrest by inhibiting S phase in colon cancer cell line, the mechanism might accomplished by targeting PHB-P53-P21 pathway.
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