With the development of molecular biology mutants are important in the biotechnology research area all over the world.Some mutants were seemed as no usefulness before are important today.Isolated microspore culture techniques have been used to generate haploid and doubled haploid (DH) lines.And these DH lines are useful in mutant selection in many species.A simple,rapid and economic method to get leaves mutants from Brassica allosextuple species CGMCC N(o) 2553 was founded in this paper.The method mainly includes two steps.