Herba Epimdii is a traditional Chinese medicine used for treatment of osteoporosis [1].The main pharmacological mechanisms were that epimedium flavonoids transformed into secondary glycosides or aglycon mediated by intestinal flora and enzymes,leading to enhanced absorption and anti-osteoporosis activities [2].However,individual difference of intestinal enzyme environment posed a real challenge in the clinic application.According to our previous studies,obviously enhanced absorption of epimedium flavonoids was observed after hydrolysis fully in vitro.