【摘 要】
：
From an environmental perspective, utilizing nonconventional solvents (i.e.green solvents) with low ecological footprint is a highly beneficial alternative to the conventional organic solvents to form
【机 构】
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State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China Uni
【出 处】
：
2015年上海市研究生学术论坛(生物化工)
论文部分内容阅读
From an environmental perspective, utilizing nonconventional solvents (i.e.green solvents) with low ecological footprint is a highly beneficial alternative to the conventional organic solvents to form the reaction system.Nitrile hydratase (NHase, EC 4.2.1.84) catalyzed hydration of 2-amino-2, 3-dimethylbutyronitrile (ADBN) to 2-amino-2, 3-dimethylbutyramide (ADBA) in various green solvent-aqueous reaction systems were investigated in this study.After systematical optimization of reaction conditions, HFE-7100/H2O (v/v, 10%) biphasic system was ultimately identified as a promising reaction system in the aspects of reducing product inhibition, avoiding substrate hydrolysis, facilitating product separation and solvent recovery.The average ADBA yield of entire batch reaction attained 97.3 %, which was obviously higher than those previously reported chemical or enzymatic methods.This is the first attempt to apply a fluorous solvent-aqueous biphasic system into a biocatalytic process, and the results suggests that proper fluorous solvent employed in the biphasic system is more accord with the requirement of green chemistry.
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