Objective: To evaluate the diagnostic value of SLN biopsy combined with FS examination in detecting nodal metastasis of cervical cancer.Methods: Seventy-five patients with FtGO stage IA2-ⅡB cervical cancer were enrolled in this prospective study SLN biopsy and bilateral pelvic lymphadenectomy were performed for each patient.All detected SLNs were assessed by intraoperative frozen section (FS) examination.Postoperative paraffin section (PS) examination included an ultra-staging protocol for SLNs with negative FS diagnosis.Results: Of the 75 patients,at least one SLN was successfully detected in 69 patients.A total of 15 metastatic SLNs were confirmed in 10 patients.Besides,one patient without SLN detected had unilateral pelvic nodal metastasis.Two patients with FS-negative diagnosis had SLN micrometastasis or isolated tumor cells (ITC) detected by serial section examination.No pelvic or paraaortic nodal metastasis was detected in patients with PS-negative SLN.On the basis of PS examination,the sensitivity and negative predictive value (NPV) for SLN biopsy were 100% (10/10,95%CI:71.7%-100.0%) and 100%(59/59,95%CI: 95.4%-100.0%),respectively,while they were 80.0% (8/10,95%CI: 40.0%-97.2%) and 96.8% (59/61,95%CI:89.0%-99.6%) on the basis of FS examination,respectively Neither the sensitivity (P=0.757) nor NPV (P=0.899) showed significant difference between the FS and PS examinations.Conclusion:The SLN biopsy combined with FS examination has acceptable accuracy in predicting pelvic nodal metastasis.In spite of its poor capacity in detecting micrometastasis and ITC,the FS examination is not significantly worse than PS one and should be performed as a routine procedure in SLN biopsy for cervical cancer.