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Objectives Excessive activation of nuclear transcription factor-κB (NF-κB) is involved in human airway smooth muscle cells (HASMCs) activities in asthma.We investigated the effects of 1,25-dihydroxyvitamin D3 [1,25-(OH) 2D3] on the NF-κB signaling pathway in passively sensitized HASMCs and the molecular mechanisms involved.