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Objective To study the antitumor effects of a traditional Chinese medicine named atractylodes macrocephala koidz volatile oil,and to search biological features and related secreted proteins expression of prostate cancer cells LNCap and DU145 in vitro.Methods Cultured cells were divided into four control groups including:Blank culture without serum and without cell (group A),Blank culture with serum but without cell (group B),LNCap cell culture with serum (group C),DU145 cell culture with serum (group D).