【摘 要】
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DNA was isolated from the sapwood,transition wood and heartwood of fresh and dried Cunninghamia lanceolata wood using two DNA extraction protocols: the modified CTAB method and the modified Qiagen kit
【机 构】
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Wood Anatomy and Utilization Department,Chinese Research Institute of Wood Industry,Chinese Academy
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DNA was isolated from the sapwood,transition wood and heartwood of fresh and dried Cunninghamia lanceolata wood using two DNA extraction protocols: the modified CTAB method and the modified Qiagen kit.Our major objective was to (i) determine an optimized method for retrieving good quality and sufficient quantity of DNA from wood,and to (ii) investigate the effect of different radial positions of fresh and dried wood for DNA extraction.In comparison with the modified CTAB method,a greater quantity of higher quality DNA was retrieved using the Qiagen kit protocol.The quantity and purity of the DNA from the sapwood and transition wood was greater than that from the heartwood.Due to the influence of the drying treatment,the quantity of DNA decreased by more than 50%.The rDNA-ITS region retrieved from sapwood using both protocols could be successfully amplified,although the PCR amplification failed for the nuclear ribosomal DNA internal transcribed spacer (rDNA-ITS) from heartwood.The optimized radial position for DNA extraction in the stem was demonstrated based on anatomical observation.
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