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The Spodoptera litura nucleopolyhedrovirus (SpltNPV) gp37 fusion gene was inserted into the transfer vector (pBacPAK9),named pBacPAKSL37fus.The vector was cotransfected BT 1-Tn5B 1-4 (Hi5) cells with liner Autographa californica nucleopolyhedrovirus(AcNPV) DNA (BacPAK6).The purified,non-occluded body recombinant baculovirus (Ac-PAKSL37fus) was selected by successive 3 cycles of plaque purification.The inserted gp37 fusion gene under the control of polyhedrin gene could high-express gp37fus protein.At 24 hours post infection (hr.p.i),sf-21 cells infected with recombinant virus had a distinctive cytopathic effect compared to cells with wild type (w.t) AcNPV.