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Kaposis sarcoma-associated herpesvirus (KSHV), also known as human herpesvirus 8 (HHV-8), is a cancer-related human virus, classified as a member of the gammaherpesvirinae subfamily.The present study describes the construction and characterization of a new dual fluorescent-tagged recombinant KSHV genome (BAC16-mCherry-ORF45), which constitutively expresses GFP and contains the tegument multifunctional ORF45 protein as a fusion protein with monomeric Cherry fluorescent protein (mCherry).