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  5. Identification of Suitable Reference Gene for qPCR Analysis of Serum microRNA in Gastric Cancer Pati

Identification of Suitable Reference Gene for qPCR Analysis of Serum microRNA in Gastric Cancer Pati

来源 :首都医科大学2013年研究生学术论坛 | 被引量 : 0次 | 上传用户：lyhmj
【摘 要】
：
Background: Circulating microRNAs expression profiles could be a promising biomarker for diagnosis, prognosis as well as therapeutic target for cancer patients.Currently quantitative real-time polymer
【作 者】
：
Jianning Song Zhigang Bai Wei Han Jun Zhang Hua Meng Jintao Bi Xuemei Ma Shiwei Han Zhongtao Zhang 
【机 构】
：
Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 1000
【出 处】
：
首都医科大学2013年研究生学术论坛
【发表日期】
：
2013年2期
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　　Background: Circulating microRNAs expression profiles could be a promising biomarker for diagnosis, prognosis as well as therapeutic target for cancer patients.Currently quantitative real-time polymerase chain reaction is a sensitive method to estimate the expression levels of circulating microRNAs.However there was no current consensus on reference gene for qPCR data analysis of circulating microRNAs.This study describes the identification and characterization of appropriate reference genes for normalization of serum microRNAs levels in gastric cancer patients and healthy people.Aims: Identify suitable reference genes for qPCR analysis of serum microRNA in gastric cancer patients.
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