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Aim To study the effects of breast tumor cells on the gene expression profiles of fibroblasts in microenvironment.Methods Fibroblasts were treated with the condition medium from MCF-7、 MDA-MB-231 and MCF-10A culture supernatants and microarray were done with mRNA from the above treatment of fibroblasts.Real-time quantitative RT-PCR was performed to test the results of microarray.Results In the fibroblasts treated by MCF-7、 MDA-MB-231 and MCF-10A tumor cell culture supernants, there were significantly up-regulated 484, 439 and 1121 genes and down-regulated 629, 587 and 1138 genes, respectively.Hierarchical cluster analysis of the gene expression profiles of the fibroblasts treated with the condition medium from MCF-7、 MDA-MB-231 and MCF-10A showed that the global gene expression patterns of fibroblasts treated with high-invasive MCF-7 and MDA-MB-231 were more similar to one another and clustered distinctly from fibroblasts treated with non-invasive MCF-10A.the gene expression profiles of the condition-cultured fibroblasts were associated with inflammatory process and the tread and fold of gene changes in fibroblasts were postively relative to the invasive and metastatic ability of MCF-7、 MDA-MB-231 and MCF-10A tumor cells.Six differentially expressed genes screened out by the microarray approach were validated with real-time quantitative RT-PCR.Conclusions Breast tumor cells can regulate the gene expression of fibroblast in microenvironment, which are associated with the invasive and metastatic ability of breast tumors themselves and help invasion and metastation of tumor cells.