论文部分内容阅读
It has been suggested that the main source of GABAergic neurons in the rodent neocortex and spinal cord is in the subpallium and the ventricular zone of the spinal cord,respectively.Our study revealed a production of GABAergic neurons in the mouse neocortex and the spinal mantle layer.Using immunohistochemistry,we detected that about 25% of the GFP-positive cells in the cortical subventricular zone (SVZ) of GAD67-GFP knock-in mouse at E 18 were positive for Ki-67 and some of them were undergoing mitosis or in the DNA synthesis phase.Dissociated cells obtained from the deeper half of the neocortex in GAD67-GFP knock-in mouse at E 18 were further used for immunocytochemistry and single-cell RT-PCR.