【摘 要】
:
In avian,vasoactive intestinal peptide(VIP)as a prolactin(PRL)releasing factor,is involved in the control of the PRL release,and plays a pivotal role as a regulator of reproductive behavior and neuroe
【机 构】
:
Institute of Animal and Veterinary Science Research,Shanghai Academy of Agricultural Sciences,Shangh
论文部分内容阅读
In avian,vasoactive intestinal peptide(VIP)as a prolactin(PRL)releasing factor,is involved in the control of the PRL release,and plays a pivotal role as a regulator of reproductive behavior and neuroendocrine secretion.In this study,a 833 bp fragment covering the complete coding region(CDS)of goose prepro-VIP gene was obtained.The cDNA contains a 32 bp 5′-untranslated region(UTR),a 306 bp CDS,and a 495 bp 3′-UTR including a TGA termination codon(nucleotides 518–520 bp)and the poly(A)tail.The goose prepro-VIP gene shares about 67–94%nucleic acid identity and 53–98%amino acid identity with their counterparts in other species.The sequence analysis indicated that two alternatively spliced transcripts were identified,the larger transcript(VIP-1)encoded both VIP and peptide histidine isoleucine(PHI)exons and the short one(VIP-2)only encoded VIP.Based on the cDNA sequence,the genomic structure of this gene was characterized.The goose prepro-VIP gene consists of 7 exons and 6 introns,just as that in other avian species.The RT-PCR analysis indicates that the level of the VIP-1 is much higher than that of the VIP-2,and the lager one negligible or absent in liver,kidney,muscle and abdominal fat,while the short one is widely distributed in all examined tissues.
其他文献
Our previous work predicted the existence of a genetically diverse and recombining source population of Pseudomonas syringae pv.actinidiae (Psa), from which stochastic sampling events followed by sele
Phytoplasma, an obligate intracellular parasite, can stimulate the plant defense response.In this paper, SWP11, a secreted protein encoded by WBD_ 0236, was identified as an elicitor.This protein coul
Both paulownia and jujube witches-broom are economically and environmentally harmful plant diseases caused by two distinct phytoplasmas belonging to 16SrI and V group respectively.
Wheat Blue dwarf phytoplasma (WBDp) disease is an important plant disea-se in the northwest region of China.It is transmitted by the leafhopper, Psammotettix striatus, in the field.The disease occurs
Morphological and molecular identification were made among 3 populations ofHirschmanniella, occurring onbitter gourd of QiongzhongMaoyang Town (QGS32), bitter gourd of QiongzhongHongmao Farm (QGS33),
引言猪高致病性蓝耳病(PRRS)、口蹄疫(MFD)都属于急性、烈性、病毒性的一类猪重大传染病,往往给养猪场和养殖户带来巨大经济损失.养猪生产中,由于各养殖场饲养猪品种不一样,在发生高致病性蓝耳病、口蹄疫等疫情时往往表现出明显的品种间差异,对猪场生产成绩影响差异也大.通过临床观测发现,在猪场发生高致病性蓝耳病、口蹄疫时,黑猪相较外种猪表现出明显的抗病力.现将观测结果报道如下.
引言产肠毒大肠杆菌(ETEC)会对新生和断奶仔猪造成严重的腹泻.葛根芩连汤是治疗湿热腹泻证的经典组方,葛根素、黄芩苷、黄连素分别是葛根、黄芩和黄连的有效活性成分.本试验以猪肠道上皮细胞(PEC-J2)为研究对象,探讨葛根素、黄芩素、黄连素和香豆素对ETEC生长情况的影响,以及药物对感染ETEC的IPEC-J2细胞形态变化的调控,旨在为临床治疗断奶仔猪腹泻开发新药物.
Three major distinct types of lysozymes have been identified in the animal kingdom and most lysozymes cloned from ruminants belong to the chicken-type(c-type).In this study,a new c-type lysozyme gene
Growth hormone(GH)has been considered as a candidate gene for growth and body size in pigs.In this study,polymorphisms of the GH1 gene were evaluated for associations with body size traits in 190 pig
Growth hormone releasing peptide-6(GHRP-6)plays an important role in animal growth.However,there was few study focused on the effect of GHRP-6 on animal growth through controlled-release system.We hav