GLP的实施水平会影响到动物实验的实施是否适当。对动物实验结果有影响的因素的恒定或设定条件的维持、控制对保证实验的重现性具有重要影响。科学的动物饲育管理,以及完善的操作记录,能够保证良好的实验质量。饲育环境的各种指标必须尽可能在基准值范围内。另外,动物从接收、检疫、治疗、实验等环节,都必须进行卫生的饲育管理。动物相关的各种数据也必须获取。实验设施中日常的动物饲育管理必须制定必要的SOP,不应该有遗
The aim of this article is to examine the toxic effects of hexabromocyclododecanes(HBCDs) on astrocytes primary culture and conditional culture solution of HBCDs-treated astrocytes on the survival of
目的 通过激光诱导猴眼底黄斑区脉络膜新生血管(CNV)渗漏和生长，建立与人脉络膜新生血管类似的动物模型，对拟用于治疗脉络膜新生血管相关疾病药物的药效学进行评价。方法 采用眼科激光光凝仪围绕猴黄斑中心凹进行光凝造模，光凝后2～3周，进行荧光素眼底血管造影检查，筛选出造模成功的动物，根据造模效果分为模型对照组、阳性对照组、药物低、中、高剂量组。分别于给药前、给药后14和28 d进行眼底彩色照相、荧光素
Green tea has a considerable amount of polyphenolic catechins and has been promoted to have beneficial effects on health including protective effects against oxidative stress.Acetaminophen (APAP) is a
Tetrandrine is an alkaloid with a remarkable pharmacological profile that we previous reported causes hepatotoxicity through mitochondrial dysfunction.Here, we demonstrate that cytochrome P450 (CYP450
OBJECTIVE Cisplatin-induced nephrotoxicity has been characterized previously in animals and humans.Two studies were conducted in cynomolgus macaques to assess the nephrotoxicity of cisplatin and to co
目的 建立DSS诱导大鼠急性炎症性肠病(IBD)模型,研究维生素D和维生素E对急性IBD的治疗作用及探讨其作用机制.方法 ①将雄性Wistar大鼠分为如下7组,正常对照,DSS模型对照,泼尼松龙(1 mg·kg.·d-1)阳性对照,维生素D(50 ng·d-1),维生素E(2 g·kg-1·d-1),维生素D(50 ng·d-1) +E(2 9·kg-1·d-1)联合,以及泼尼松龙(1 mg·kg
目的 通过研究可卡因成瘾小鼠伏隔核转录本表达情况，探索可卡因成瘾中长链非编码RNA作用机制，为将来研究长链非编码RNA在药物成瘾中作用提供一个良好的信息资源平台。方法 建立可卡因条件性位置偏好小鼠模型，通过高通量芯片研究伏隔核脑区转录本的差异化，并利用生物信息学方法对分析差异表达的信使RNA进行GO分析，对长链非编码RNA进行基因组背景分析和cis-/trans-调控分析。最后对生物信息学表达结果
The application of strychnine (S) is limiteddue to its toxicity, but its derivative strychnine nitrogen oxide (SNO) is used widely in clinical practice.We here employedZebrafish embryos to investigate
OBJECTIVE To evaluate the genotoxicity of Descarboxyl Levofloxacin by In silico and In vitro methods.METHODS Assessment of genotoxicity using Derek for Windows We used DEREK for Windows (DfWV, Lhasa L