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Chrysanthemum, a traditional Chinese medicine, shows various pharmacological activities including anti-inflammatory, anti-oxidative and free radical scavenging.It has long been extensively used for removing liver-fire and improving eyesight in Chinese medicinal formulae.We presumed that chrysanthemum may affect vitamin A (retinol) metabolism since retinol plays a key role in the visual cycle and the vision maintains.The purpose of this study was to verify the hypothesis.