【摘 要】
：
Objective To find whether D-Tryptophan,D-Leucine,D-tyrosine,and D-Methionine have effect on the biofilm formation by Porphyromonas gingivalis (P.gingivalis) W83.Method P.gingivalis W83 were cultured i
【机 构】
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Dept.of Periodontics, School of Stomatology, China Medical University, Liaoning Institution of Denta
【出 处】
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第四届国际人体微生态大会、第十三届中华预防医学会微生态学学术会议、第五届中国医师协会感染科医师大会暨传染病诊治高峰论坛、
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Objective To find whether D-Tryptophan,D-Leucine,D-tyrosine,and D-Methionine have effect on the biofilm formation by Porphyromonas gingivalis (P.gingivalis) W83.Method P.gingivalis W83 were cultured in Trypticase soy broth (TSB) supplemented with vitamin K (1μg/ml) and hemin (5 μg/ml) to mid-log phase,diluted 1:100 in 96 well flat-bottom poltstyrene plates and add D-Tryptophan (5mM),D-Leucine (8.5mM),D-tyrosine (3 μM),and D-Methionine (2mM).The biofilms were stained with 25 μ 1 of 1% crystal violet,washed with ddH2O and disolved in ethanol.The absorbance was measured with xMark microplate spectrophotometer at 570nm.
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