Salivary gland adenoid cystic carcinoma(AdCC)of the head and neck is characterized by indolent while persistent growth,multiple local recurrences and early distant metastasis.EGF receptor(EGFR)is deemed to have pivotal roles in AdCC progression.This study aims to investigate the correlation between angiogenesis and EGFR signaling pathway.The expression of EGFR,CD31,HIF-1α,and CD 146 was evaluated by immunohistochemical staining using tissue microarray(TMA)containing normal salivary gland(NSG),pleomorphic adenoma(PMA)and AdCC tissues.Pearson correlation analysis was conducted to explore the correlation among EGFR,CD146,HIF-1α and CD31.For their similarity and intimacy,hierarchical analysis was performed with Cluster 3.0 and visualized using Java TreeView.Quantification for the immunohistochemistry of TMA revealed that the expression of EGFR,CD146,HIF-1α increased in AdCC as compared with PMA and NSG.The relationships among expression of EGFR and CD31 were significantly positively correlated.Expression of CD146 and HIF-1α were positively correlated with both EGFR and CD3 i.Collectively,these findings suggested the vital roles of EGFR signaling pathway in AdCC progress through their relation with HIF-1 α and CD 146-mediated angiogenesis,which provided further understandings of the mechanism during the progression of AdCC as well as potential clinical significances.