Introduction: To investigate the correlation between clinical features of ankylosing spondylitis(AS)and different Human leukocyte antigen(HLA)-B27 subtypes.Method: We conducted a cross-sectional study of 216 patients with AS.HLA-B27 and its subtypes were detected by polymerase chain reaction with sequence specific primer(PCR-SSP).Clinical features were compared between the different HLA-B27 subtypes.A meta-analysis on uveitis frequencies in AS patients with HLAB*2705 vs 2704 was performed.Results: The most prevalent subtypes of HLA-B27 were HLA-B*2704(66.1%)and HLA-B*2705(32.2%).There were 57 HLA-B27 positive AS patients with the history of uveitis,45 were B*2704 and 12 were B*2705,patients with B*2704 had more uveitis than B*2705(p=0.021).After meta-analysis,there was no significant difference in the presence of uveitis between HLA-B*2704 and HLAB*2705.Conclusion: AS patients with B*2704 have higher risk of uveitis than AS with B*2705 in north Chinese people.