烟草是重要的经济作物,其质量和产量受到烟草病虫害等一些重要因素的影响,烟草根腐病是农业生产上的一种重要根部病害,而耕作制度和栽培方式的改变,更是加重了该病害的发生.本文从烟草根腐病病样根部,采用常规组织分离法分离并纯化获得两种病原,通过形态学和分子生物学方法鉴定并确定两种病原菌分别为尖孢镰刀菌(Fusarium oxysporum)和黄色镰刀菌(Fusarium culmorum),并探究了两种病
Botryosphaeria dothidea is a destructive pathogen of apple and pear.To investigate the infection process,binary vectors containing various lengths of H3 promoters and TEF promoters fused with GFP and
Ustilaginoidea virens (Cooke) Tak.is the causal pathogen of rice false smut (RFS) dis-ease.RFS is an emerging panicle disease,threatening rice production worldwide.
根腐病是影响根茎类中药材产量和质量的主要因素之一。本研究采集河北唐山地区发病的柴胡植株,分离其病原菌,从病原菌的培养性状和形态学特征、内转录区(ITS)系列分析等方面对病原菌进行了鉴定;用回接法检测病原菌的致病性。
Autophagy,a ubiquitous intracellular degradation process,is conserved from yeast to human.It serves as a major survival function during nutrient depletion stress and is crucial for proper growth and d
MicroRNAs (miRNAs) are kinds of conserved small RNAs existed in both prokaryote and eukaryote.
In recent years,research has shown that microRNAs involved in regulating the resist-ance to rice blast fungus Magnaporthe oryzae in rice,but research focusing on a single miRNA is rarely reported.
钙/钙调素依赖性蛋白激酶(calcium/calmodulin dependent protein kinases,CaMKs)是Ca2+途径中主要的组成成分之一。本实验室前期已经通过克隆方法,获得CaMKs的基因,并初步利用简并引物从玉米大斑病菌中克隆到4个CaMKs基因片段,分别命名为CAK1、CAK2、CAK3和CAK4并对其进行了基因家族的鉴定和表达模式的分析。研究发现CAK3在功能表达方
核盘菌(Sclerotinia sclerotiorum(lib.)de Bary)是一种寄主范围广泛、危害极其严重的腐生型植物病原真菌.在病害循环中,适宜条件下菌核萌发形成子囊盘,成熟子囊盘可释放大量子囊孢子,子囊孢子随风散落在寄主植物上萌发并侵染,引起植物菌核病并造成病害流行,严重影响作物产量和品质.