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A large number of microbial strains were isolated from the sponge Gelliodes camosa, in which four strains were preliminarily identified as Nectria sp.(HLS-206), Hypocreales sp.(HLS-104), Penicillium sp.(HLS-216) and Penicillium chrysogenum(HLS-111) by the molecular phylogenetic analysis.Primary pharmacological screening showed the anti-tumor and/or anti-bacterial activities of the four strains.