Establishment and Applied Analysis of Determination Conditions for Cellulase Activity in Volvariella

来源 :The 7th Meeting of Asia for Mushroom Science(第七届亚洲食用菌学术会议) | 被引量 : 0次 | 上传用户：nmhnhjcf

【摘要】

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【作者】

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Zhao Yan Zhou Huimin Li Yapeng Wang Hong Chen Mingjie

【机构】

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Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences; Key Laboratory of Edible Fungi

【出处】

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The 7th Meeting of Asia for Mushroom Science(第七届亚洲食用菌学术会议)

【发表日期】

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2013年4期

【关键词】

：

Volvariella volvacea cellulase activity growth

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　　This study was aimed to optimize the concentration of microcrystalline cellulose and culture time for Volvariella volvacea strain V23 in liquid basal medium.It was found that the activity of cellulase was highest when the concentration of microcrystalline cellulose was 1%.Under 1% concentration of microcrystalline cellulose, V23 was cultured for different days to measure the cellulase activity.Results showed that V23 had the highest activity of cellulase on the 5th day.Strains V23 and A8 with different yield were cultured under the above conditions, and the growth speed and enzyme activities were measured, respectively.Results indicated that V23 mycelium grew significantly faster than A8 on cotton hulls medium.The enzyme assay results showed that cellulase activity in V23 was significantly higher than in A8.

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Establishment and Applied Analysis of Determination Conditions for Cellulase Activity in Volvariella

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