In this study, three full-length manganese peroxidase (MnP) genes from white-rot fungus Trametes gibbosa, termed Tgmnp1, Tgmnp2, and Tgmnp3, were cloned and sequenced.The predicted open reading frames (ORFs) are 1,098-, 1,095-, and 1,101-bp long and all be interrupted by five introns.The deduced amino acid sequences encode manganese peroxidases containing 365, 364, and 366 residues, respectively.The MnP gene Tgmnpl shares highest similarity (84%) to mnp from Trametes versicolor (BAB03464), Tgmnp2 reveals 88% similarity to mnp from T.versicolor (CAG33918), and Tgmnp3 showed the highest identity (89%) to MnP from T.versicolor (BAB03464).Consistent with the peroxidase mechanism of MnP, the proximal histidine, the distal histidine and the distal arginine, and the aromatic binding site (LN/I-P-X-P) are conserved.The intron splice junction sequences as well as the putative internal lariat formation sites in three mnps adhere to the GT-AG and CTRAY rules, respectively.The 5 upstream and 3 downstream non-coding flanking regions of three nnp genes were also amplified using genome walking-PCR, respectively.All promoters contain putative TATAA elements and some inverted CCAAT element as well.Further more, SP-1, AP-1, AP-2 sites and several putative metal response and heat-shock elements (MRE and HSE) were identified in each sequence.