In this paper,in order to evaluate the influence of the water FBE 103 on the water of higher-altitude Litopenaeusvan-nameiculture ponds,research was conducted by detecting the density of phytoplankton,pH and the contents of dissolved oxygen,ammonia nitrogen and nitrite content.The results showed that its phytoplankton,pH,dissolved oxygen values varied in the range of 35 x 107cell/L～ 11 × 107 cell/L,7.8～8.6,6.0～ 9.0 mg / L,respectively,and in control group the values were 22×107 cell/L～7× 107 cell/L,7.6～8.3,and 4.0～ 7.0mg/L,respectively.Both the average ammonia nitrogen and nitrite numerical values were lower than in control pool and reduced following the phytoplankton increasing.The yield of Southern American prawn increased 250kg per mu during three months of aquiculture.Hence,the application of water FBE103 can improve water quality,reduce the cast of chemical agent,be beneficial to promote the development of eco-fisheries and the pollution-free aquaculture,keep the discharge of water from shrimp ponds within the limits set by the State and increase farmer incomes.