论文部分内容阅读
Cell adhesion and migration are key cell behaviors during gastrulation,and plays a crucial role in shaping the body plan in vertebrates.Collagen triple helix repeat containing 1(cthrc1)is a novel secreted protein expressed in injured and diseased arteries,it is glycosylated and highly conserved from lower chordates to mammals.Cthrc1-overexpressing fibroblasts and smooth muscle cells show enhanced migratory ability.However,the mechanism by which it regulates cell migration and its implication during early development is not clear.Here we show that cthrc1 interacts with ?1-containing integrin to regulate cell adhesion and early morphogenetic movements during zebrafish gastrulation.Loss-of-function of maternally supplied cthrc1 specifically prevents the directed cell migration in the embryo and strongly decreases cell adhesion on fibronectin(FN)-coated substrate.Furthermore,cthrc1 functionally and biochemically interacts with ?1-containing integrin during gastrulation and is required for actin filament organization and the protrusive activity of migrating cells.These results suggest that cthrc1 may be a novel ligand of integrin,they establish a functional link between cthrc1 and integrin and provide novel mechanistic insight of how cthrc1 regulates cell adhesion and migration.They may also have relevance to metastasis and other human diseases.