【摘 要】
：
As a novel delocalized lipophilic cation (DLC),F16 shows a broad spectrum of antiproliferative action toward cancer cell lines.PVI is the precursor compound of F16 with similar structure but no positi
【机 构】
：
State Key Laboratory of Virology & Key Laboratory of Analytical Chemistry for Biology and Medicine (
【出 处】
：
中国化学会第三届全国生物物理化学会议暨国际华人生物物理化学发展论坛
论文部分内容阅读
As a novel delocalized lipophilic cation (DLC),F16 shows a broad spectrum of antiproliferative action toward cancer cell lines.PVI is the precursor compound of F16 with similar structure but no positive charge.For the purpose of investigating the influence of positive charge on mitochondrial toxicity of F16,certain parameters of mitochondrial structure and function in the presence of F16 or PVI were monitored by isolated rat liver mitochondria,combining with cell assays.It was found that F16 induced obvious toxicity on both mitochondria and cancer cells,while for PVI,in spite of isolated mitochondria and cultured cells were more resistant to this small molecule,PVI still performed toxicity on mitochondria to a certain degree.The less toxicity of PVI was attributed to the disability of accumulating in mitochondria as exhibited in colocalization assay.The results demonstrate that positive charge is an important factor for mitochondriotoxic and antiproliferative properties of F16,while the vinylindole and pyridine/pyridinium moieties also make some contribution.This study may be beneficial to the design and optimization of DLCs.
其他文献
Chemical cross-linking provides an effective avenue to reduce the conformational entropy of polypeptide chains and hence has become a popular method to induce or force structural formation in peptides
More and more evidences have shown that many biological important processes are related to the conformation and assembly state of specific gone,involved proteins and enzymes.In this report,we summariz
There are different types of membrane proteins in a cellular membrane,and most of them must correctly assemble into appropriate clusters for their cellular functions.In this work,we suggest a physical
双模态影像技术(如MR/CT、PET/CT等)能进一步提高疾病诊断的准确度.已经有文献报道采用Gd螯合剂修饰的纳米金颗粒可以实现CT/MR双模态造影[1-3].发展新型的、价廉易得的多功能纳米造影剂仍然是目前影像诊断学和纳米医学研究的热点.在本研究中,我们采用聚乙二醇(PEG)和DOTA(一种钆螯合剂)部分修饰的超支化聚乙烯亚胺(PEI)高分子为基础平台,通过在其内部包裹合成纳米金颗粒用于CT成像
MD simulation of the polyalanine,17-residue helix (pdb:2I9M) and leucine zipper are carried out based on two set of atomic charges,the standard AMBER charge and the dynamically adjusted polarized prot
A combined QM/MM protocol for the simulation of resonance Raman spectra of protein and peptides has been proposed and implemented [1].The divide and conquer strategy was applied to incorporate the loc
高度有序的多肽链纳米结构的形成是分子间各种相互作用协同的结果.我们采用粗化模型和蒙特卡罗模拟方法研究了多肽链侧链间的疏水相互作用强度对其自组装结构的影响.发现随着疏水相互作用强度和温度的变化,多肽链可以形成各种自组装结构,展现出丰富的相行为.这表明多肽链自组装结构和形貌可以通过改变疏水作用强度和温度来调控.我们的模拟结果可以为设计基于多肽链纤维结构的新型纳米生物材料提供理论依据.
Using molecular dynamics simulation technique,we compared several commonly used ion-water models on describing the microscopic structure and dynamic in the KSCN aqueous solutions.Results are compared
吡虫啉是一种内吸传导性杀虫剂,药效表现为昆虫神经毒素.它属于一类被称为新烟碱的化学药品,其作用于昆虫的中枢神经系统,而对哺乳动物表现出较低毒性[1].在模拟人体生理条件下,综合利用荧光光谱、紫外吸收光谱、圆二色谱和分子模拟等方法,研究了吡虫啉(IMI)和人血清白蛋白(HSA)的相互作用.荧光光谱和紫外吸收光谱的分析表明：吡虫啉能有效猝灭HSA的内源荧光,Stern-Volmer猝灭常数Ksv和有效
Oxoisoaporphine (1-azabenzanthrone) alkaloids were extracted from traditional Chinese medicine and used as an analgesic and antipyretic.Nowadays some analogs of oxoisoaporphines also used as antitumor