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Aqueous extract of Trametes robiniophila murr(Huaier)has been applied for cancer complementary therapy in recent decades.Varies studies have reported that Huaier possess the anti-tumor effects.However,the mechanisms are not completely elucidated.MicroRNAs (miRNAs) are small (18-25 nucleotides),noncoding RNAs whose dysregulation have been discovered to involve in tumorigenesis and development.In this study,we found miRNA expression profiles were altered in Huaier-treated human pulmonary adenocarcinoma A549 cells.