Build a method to rapid detect commercial sterilization of fungus cans.Evaluate the specificity,sensitivity and applicability of BacT/ALERT 3D system used in detection commercial sterilization of fungus cans.Build a method used the BacT/ ALERT 3D system and compared with the traditional method.The method is authentic and sensitive; it is easy,quick and only need three days to detect by this method.BacT/ALERT 3D system is applicable to detect commercial sterilization of fungus cans.