To study the dynamic variation of protein components and contents in Antheraea pernyi,2D-PAGE,in this job,was used to investigate embryo development.Some special protein spots expression profiles changed obviously were analyzed by mass spectrometry.2D-PAGE results showed that the protein spots increased from 370 at the development stage of 12 h to 422 at 300 h.The matching rates of protein spots at 132 h and 276 h of embryo development with 36 h were 52.4% and 28.4% respectively.During the early stage (12~60 h) of embryo development,numbers of protein species were relatively low which did not change obviously.While during the late stage (228~276 h),the protein species changed obviously,the acidic protein greatly increased,and the matching rate of protein spots declined.Eighteen special protein spots were identified by LC-MS/MS,and 8 kinds of proteins are found to include heat shock proteins families,Vitellogenin,KK-42-binding protein,actin-depolymerizing factor 1,etc.,which are mainly involved in some important processes.Analyze the function of those proteins,so as to accumulate basic information on embryonic development in A.pernyi.