【摘 要】
：
Selective enrichment or removal specific proteins prior to analysis can minimize nonspecific interferences as well as information loss, which has been an important issue in current proteomics fields.I
【机 构】
：
Department of Chemistry, Lanzhou University, Lanzhou 730000, China
【出 处】
：
第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会
论文部分内容阅读
Selective enrichment or removal specific proteins prior to analysis can minimize nonspecific interferences as well as information loss, which has been an important issue in current proteomics fields.In this work, a novel bifunctionalized SBA-15 with different pore size (5 nm or 7 nm) has been successfully prepared.The functionalized SBA-15 was used to investigate the adsorption behavior for different proteins (bovine serum albumin (BSA), ovalbumin (OVA), hemoglobin (Hb), lysozyme (Lys) and cytochrome c (cyt c).As possessing anion exchange (quaternary amine) groups, the bifunctionalized SBA-15 showed selective adsorption of the negative charged proteins BSA and OVA.
其他文献
Environmental pollutions and food contaminations are biggest social concerns, nowadays.Because of the complex matrices in environmental substances and food stuffs, sample preparations for the quantita
A green analytical method for the determination of parabens in cosmetic products by subcritical water chromatography (SbWC) with salt-assisted homogenous liquid liquid extraction (SHLLE) was investiga
Proteolysis is one of the key techniques of proteomics research.The development of immobilized enzyme reactors (IMER) enables the proteolysis fast and efficient.Studies show that kinds of hydrophilic
Magnetic solid phase extraction (MSPE) is based on the use of uniform Fe3O4 as well as various modified by carbon materials (C8, C18, CNTs etc.), polymer (PMMA, Polystyrene, polyaniline etc.), metal o
Molecularly imprinted polymers (MIPs) have aroused extensive attention and been widely applied in many fields, such as solid phase extraction and chemical sensors, owing to their desired selectivity,
Ethyl carbamate (EC) occurs in various alcoholic beverages and fermented foods during their fermentation.The complex matrix of food sample makes pretreatment for determination an indispensable procedu
Multiple headspace solid-phase microextraction (MHS-SPME) combined with gas chromatography-nitrogen phosphorus detector is proposed to determine the toxic contaminant ethyl carbamate (EC) in various a
Porous polymers have been accepted as an effective material for high specific surface area in favor of mass transfer1, 2.The hollow porous molecularly imprinted polymers (MIPs) were prepared by multi-
Multiple headspace-SPME (MHS-SPME) is a modification of SPME developed for quantitative determination of volatile compounds, especially in solid samples.This technique involves sampling repeatedly the
N-glycosylation is one of the most common and complex post-translational modification of proteins, which plays an important role in a variety of biological activities, i.e.receptor-ligand interaction,