Ovarian cryopreservation by vitrification is a very effective method for preserving femalesfertility during chemotherapy and radiotherapy, and studying of the gene expressionduring ovarian cryopreservation by vitrification is a common method for exploring themechanism of ovarian cryopreservation by vitrification, but most of reference gene lackingtissue-specific stability with real-time quantitative reverse transcription PCR (RT-QPCR), itis one of the most accurate and widely used techniques for expression profiling of selectedgenes.Therefore, in this study, the stability ofABL1, ACTB, CDKN1A, GPER, GAPDH, GUSB,HPRT1, HSP90AB, IPO8, PPIA, RPL30, RPL4, TBP and uPAR were analyzed by GeNorm,NormFinder,BestKeeper, and the Delta CT during on ovarian tissue cryopreserved by vitrificationin mice.Through the studying the stability of reference gene with real-time PCR toensure accurate reference genes were selected for normalizing the gene expression.