Objective: To compare the difference of tumor growth between CTVT cell suspension group and tumor fragment group so as to optimize the fabrication method of animal model. Methods:Using 20 beagle dogs, 10 for cell suspension group and 10 for tumor fragment group, cell suspension and tumor fragment was transplanted into lungs under CT guidance. Results: 7 of the 10 dogs was detected the formation of tumorfor cell suspension group. Ten weeks after inoculation the formation of tumor was detected in 10 inoculated sites with inoculation success rate was 62.5%. The formation of tumor can be detected in 13 inoculated sites of 10 dogs in tumor fragment group with inoculation success rate was 100%. The general condition of dogs in tumor fragment group are better than that of cell suspension group, no cachexia was detected in tumor fragment group. Nolethal complications occurred in both groups. Conclusion: Tumor formation rate of tumor fragment group was higher than cell suspension group. The incidence rate of pleural effusion and metastasis is lower in tumor fragment group than what is in cell suspension group. The general condition of dogs in tumor fragment group is better and tumor fragment inoculation is more suitable for the follow-up researches for intervention therapy.