Objectives : The search for a suitable material to guide the nerve regeneration has been a challenging tasks.Many kinds of materials including artificial or natural products has been studied for this purpose,despite its limitations and complications.We reviewed the current trends of investigation using various natural,artificial or combined biomaterials.This presentation include the results of our research for peripheral nerve regeneration using conduit consisting with porcine small intestinal submucosa(SIS),PLCL(combination of PGA and poly-caprolactone)and PLCL-SIS combination materials.Material and Methods : All experiments were done with rat sciatic nerve trans-section model.PLCL and PLCL combination nerve conduit were made with 3D printing technology.Silicone was used as control.Motor function test,electromyography study and histologic analysis were done for evaluating the results.Results : SIS conduit ! was good for the regeneration of peripheral nerve regeneration,but it was too weak for long segment nerve graft.PLCL conduit was easy to make but less regeneration activity and more inflammation.PLCL-SIS combination conduit was good for regeneration and less inflammation.3D printing technology could give more effective structure for regeneration activity of conduit Conclusion : These results show that PLCL-SIS conduit was good option as a natural-synthetic combined biomaterial for peripheral nerve regeneration.And more,it could be used as biocompatible barriers covering neural tissue,for example,dural repair,since this material contains many kinds of nerve growth factors.