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Objective: To analysis the transcriptional expression of Zonula Occluden-1 gene and the protein expression in oral verrucous carcinoma(OVC)and oral squamous cell carcinoma(OSCC),inquire into ZO-1 related epithelial–mesenchymal transition in the development of oral cancer.Analysis the relationship between ZO-1 expression with the clinic and pathology subtypes of oral cancer.