RNA inference (RNAi) technology achieved good results in hepatitis B virus (HBV) treatment in vitro.But there are challenges for long-term suppression of HBV replication.In the present study,we designed a series of recombination HBVs (rHBVs) that express no viral protein and contain certain sequence deletions substituted by a short hairpin RNA (shRNA)-expression cassette targeting the deleted sequence for HBV suppression.