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The steroidal hormone brassinosteroids(BRs)take on critical roles in numerous plant development processes.A fully connected BR signaling pathway has been elucidated in Arabidopsis by genetics,genomics and proteomics studies.However,these conventional methods can not report the transduction process in situ or resolve details at a rate matching the true signaling time-scale.Here we use a single-molecule assay to observe the interaction of GRF8,BES1 and BIN2 in BR signaling pathway.