锦鲤鳍条组织细胞系的建立及其对病毒的敏感性

来源 :中国水产学会鱼病学专业委员会2011年学术讨论会 | 被引量 : 0次 | 上传用户:zhongxuhong
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  采用组织块移植培养技术,对来源于锦鲤(Cryprinus carpiod)鳍条组织的细胞进行原代培养,建立了锦鲤鳍条组织细胞系,命名为Koi-Fin,该细胞系已稳定传代60多代.锦鲤鳍条组织细胞为成纤维样细胞,最佳培养基为MEME,最适培养温度为25℃,最适血清体积分数为10%,细胞群体倍增时间为43.5h.
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