【摘 要】
：
Biological nitrogen fixation is highly controlled at the transcriptional level by regulatory networks that respond to the availability of fixed nitrogen.In
【机 构】
：
BiotechnologyResearchInstitute,ChineseAcademyofAgriculturalSciences,KeyLaboratoryofCropBiotechnology
【出 处】
：
2rd International Symposium on Integrative Plant Biology第二届国
论文部分内容阅读
Biological nitrogen fixation is highly controlled at the transcriptional level by regulatory networks that respond to the availability of fixed nitrogen.In many diazotrophs, addition of excess ammonium to the growth medium results in immediate repression of nif gene transcription.Pseudomonas stutzeri A1501 was isolated from rice paddy soils and has been widely used as a crop inoculant in China.Availability of the A1501 genome sequence is of fundamental importance to our understanding of the evolution of the nitrogen fixation property, as well as for identification of rhizosphere competence traits required in interactions with host plants.The genome consists of a single circular chromosome with 4,567,418 base pairs.Among the 4,146 protein-encoding genes, about 2,000 of these have orthologs in each of five representative Pseudomonas species genomes.All the genetic information specific to nitrogen fixation is clustered in the A1501 genome in a 49 kb nitrogen fixation region consisting of 59 ORFs.Global transcriptional profiling analysis of nitrogen fixation and ammonium repression in A1501 reveals that a total of 166 genes, including those coding for the global nitrogen regulation (Ntr) and Nif-specific regulatory proteins, were upregulated under nitrogen fixation conditions but were rapidly downregulated as early as 10 min after ammonium shock.
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